Breast cancer cells use forces to open up channels through tissue

An illustrated microscope view of a 3D culture of cancer cells. A cancer cell generates forces (in red) moving the tissue material farther. The new technique detects the material movement to compute cellular forces.
Image Credit: Juho Pokki/Aalto University

Research to understand how cancers grow and spread has conventionally been done on two-dimensional, flat cultures of cells, which is very different to the three-dimensional structure of cells in the body. 3D cell cultures that incorporate tissue material have been developed, but the methods to measure how cancer cells use forces to spread have been lacking.

Now, researchers have developed a new method for 3D culture to accurately quantify how cancer cells generate forces to spread within tissue. ‘We have applied the method for investigation of early progression of breast cancer,’ says Juho Pokki, a principal investigator at Aalto University who led the research.

This study, a collaboration between scientists at Aalto University and Stanford University, was published in the journal Nano Letters.

Mini donkey gets big boost from pacemaker

Veterinarians at Cornell prepare miniature donkey Nix for her pacemaker surgery.
Credit: Carol Jennings/CVM 

Nix, a miniature donkey with a potentially fatal heart condition, is on the mend after a successful pacemaker implantation by veterinarians at the Cornell University Hospital for Animals – the first surgery of its kind in a large animal species at Cornell.

“It was either do nothing and Nix would continue to get worse and possibly have a painful death – or the pacemaker,” said Mindy Lockwood of Canandaigua, New York, who owns Nix with her husband, Carlton.

Nix’s collapsing episodes and overall lethargy began in the fall of 2020 when she was only a few months old. Her regular veterinarian, Dr. Joan Ayers of Genesee Valley Veterinary Hospital, assessed her condition in consultation with the Lockwoods and Dr. Barbara Delvescovo, clinical fellow in the Section of Large Animal Medicine at Cornell.

In February of this year, Nix’s condition worsened. The Lockwoods saw that Nix was falling again, this time from a standing position, and she staggered even more while walking. “When she fell, she was dazed for a few seconds and then would get back up. Several times she fell and rolled out of the pasture fence, which caused us even more concern for her safety,” Carlton Lockwood said.

Uncovering the Past: Researchers Create 3D Images of Fossils

Aase mounts one of the fossils in the X-ray microscope for imaging.
Credit: Idaho National Laboratory

Idaho National Laboratory is perhaps best known for innovative research that helps shape the clean energy economies of today and tomorrow – and for good reason. But while much of the laboratory’s work is focused on building a sustainable future, INL is also doing its part to preserve the past.

INL researchers recently imaged several fossils using a powerful X-ray microscope. The 3D images will be used to create exhibits for Wyoming’s Fossil Butte National Monument and help experts gain insight into the origins of these and other relics.

The fossils, found in private quarries around Wyoming, were imaged using a technique known as X-ray microscopy. At INL, researchers typically use high-resolution X-ray microscopy to view specimens – such as samples of irradiated nuclear fuel — at a level of detail not possible with conventional microscopes. The depth and granularity afforded by this technique will help paleontologists learn a great deal about these fossils —including an unknown object resembling an insect egg case or pea pod — and the conditions under which they formed.

“You can see the limestone layers as well as submillimeter and thinner organic materials that have been compressed into waxy, pre-petroleum substances around the specimen,” said Arvid Aase, a paleontologist and the museum curator at Fossil Butte National Monument. “These incredibly detailed images will help us determine the organism’s taxonomy and reveal information about its fossilization process, such as how long it was laying on the bottom of the lake covered in microbes before getting buried by limestone.”

The fossilization process may have occurred over a period of months, though the timing still remains unknown, he added.

Northwestern rocket to image supernova remnant

A Northwestern University astrophysics team is aiming for the stars — well, a dead star, that is.

On Aug. 21, the NASA-funded team will launch its “Micro-X” rocket from White Sands Missile Range in southern New Mexico. The rocket will spend 15 minutes in space — just enough time to snap a quick image of supernova remnant Cassiopeia A, a star in the Cassiopeia constellation that exploded approximately 11,000 light-years away from Earth. Then, the rocket will parachute back to Earth, landing in the desert — about 45 miles from the launchpad — where the Northwestern team will recover its payload.

Short for “high-resolution microcalorimeter X-ray imaging rocket,” the Micro-X rocket will carry a superconductor-based X-ray imaging spectrometer that is capable of measuring the energy of each incoming X-ray from astronomical sources with unprecedented accuracy.

“The supernova remnant is so hot that most of the light it emits is not in the visible range,” said Northwestern’s Enectali Figueroa-Feliciano, who leads the project. “We have to use X-ray imaging, which isn’t possible from Earth because our atmosphere absorbs X-rays. That’s why we have to go into space. It’s like if you jumped into the air, snapped a photo just as your head peeked above the atmosphere and then landed back down.”

Figueroa-Feliciano is a professor of physics and astronomy in the Weinberg College of Arts and Sciences and a member of Northwestern’s Center for Interdisciplinary Exploration and Research in Astrophysics (CIERA). He advised a team of seven graduate students, postdoctoral fellows and post-baccalaureate researchers, who spent the past decade building and testing the rocket.

MIT scientists discover new antiviral defense system in bacteria

A team led by researchers at the Broad Institute of MIT and Harvard and the McGovern Institute for Brain Research at MIT has discovered that organisms across all three domains of life — bacteria, archaea, and eukaryotes (which includes plants and animals) — use pattern recognition of conserved viral proteins to defend against pathogens.
Credits: Image courtesy of Feng Zhang

Bacteria use a variety of defense strategies to fight off viral infection, and some of these systems have led to groundbreaking technologies, such as CRISPR-based gene-editing. Scientists predict there are many more antiviral weapons yet to be found in the microbial world.

A team led by researchers at the Broad Institute of MIT and Harvard and the McGovern Institute for Brain Research at MIT has discovered and characterized one of these unexplored microbial defense systems. They found that certain proteins in bacteria and archaea (together known as prokaryotes) detect viruses in surprisingly direct ways, recognizing key parts of the viruses and causing the single-celled organisms to commit suicide to quell the infection within a microbial community. The study is the first time this mechanism has been seen in prokaryotes and shows that organisms across all three domains of life — bacteria, archaea, and eukaryotes (which includes plants and animals) — use pattern recognition of conserved viral proteins to defend against pathogens.

The study appears in Science.

Large number of stem cell lines carry significant DNA damage, say researchers

Our Sun Credit:
Alexas Fotos / Public Domain

DNA damage caused by factors such as ultraviolet radiation affect nearly three-quarters of all stem cell lines derived from human skin cells, say Cambridge researchers, who argue that whole genome sequencing is essential for confirming if cell lines are usable.

Stem cells are a special type of cell that can be programmed to become almost any type of cell within the body. They are currently used for studies on the development of organs and even the early stages of the embryo.

Increasingly, researchers are turning to stem cells as ways of developing new treatments, known as cell-based therapies. Other potential applications include programming stem cells to grow into nerve cells to replace those lost to neurodegeneration in diseases such as Parkinson’s.

Originally, stem cells were derived from embryos, but it is now possible to derive stem cells from adult skin cells. These so-called induced pluripotent stem cells (iPSCs) have now been generated from a range of tissues, including blood, which is increasing in popularity due to its ease of derivation.

However, researchers at the University of Cambridge and Wellcome Sanger Institute have discovered a problem with stem cell lines derived from both skin cells and blood. When they examined the genomes of the stem cell lines in detail, they found that nearly three quarters carried substantial damage to their DNA that could compromise their use both in research and, crucially, in cell-based therapies. Their findings represent the largest genetic study to date of iPSCs and are published today in Nature Genetics.

DNA is made up of three billion pairs of nucleotides, molecules represented by the letters A, C, G and T. Over time, damage to our DNA, for example from ultraviolet radiation, can lead to mutations – a letter C might change to a letter T, for example. ‘Fingerprints’ left on our DNA can reveal what is responsible for this damage. As these mutations accumulate, they can have a profound effect on the function of cells and in some cases lead to tumors.

Genome Editing Terminology Is Standardized in NIST-Led Effort

These words are included in the new Genome Editing Vocabulary. 
Credit: N. Hanacek/NIST

Genome editing can cure diseases, boost food production and open vast new fields of scientific discovery. But to realize its full potential, scientists need to precisely describe the details of their genome editing attempts to one another and the wider world.

For instance, if a company is developing a new gene therapy for use in the United States, it needs to tell the U.S. Food and Drug Administration (FDA) what the product does and demonstrate that it is safe and effective. Scientists could do that more precisely if they had a standard set of terms and definitions.

As of recently, they have one. In November 2021 the International Organization for Standardization (ISO) published the Genome Editing Vocabulary — an internationally agreed-upon list of 42 precisely defined terms that will help scientists from all over the world avoid errors of communication. (The word “genome” refers to all the inherited DNA in an organism.)

This effort was spearheaded by the National Institute of Standards and Technology (NIST) Genome Editing Consortium — an international group of industry, academic and government scientists who work in this field. NIST first convened the consortium in 2018 so that experts and organizations that often compete with one another would have a venue for collaborating on standards that advance the field for all. The FDA joined the consortium last year.

Do you keep secrets from your loved ones, family, or friends?

Ninety percent of people have kept an everyday consumer decision a secret from a spouse or other close relationship 
Photo Credit: Noelle Otto

It turns out that many people do. Whether ordering something online and hiding the package when it arrives, hiring a cleaning service and not telling your roommate, or eating a pizza instead of dieting, we often have secret purchases that we just prefer not to divulge.

UConn marketing professor Danielle Brick is investigating this behavior and discovering the little “errors of omissions” that go on in many households.

“I think what makes this research important, and fun, is how relatable it is,’ says Brick, a new member of the UConn School of Business faculty.

Her research, titled “Secret Consumer Behaviors in Close Relationships,” has just been published in the Journal of Consumer Psychology.

She and her colleagues found that this clandestine behavior not only impacts relationships in an unexpected and significant way, but also has considerable marketing implications.

Nearly a hundred genes have been lost during the woolly mammoth’s evolution

Tusk from woolly mammoth emerging from the permafrost on Wrangel Island.
Photo Credit: Love Dalén/Stockholm University.

A new study shows that 87 genes have been affected by deletions or short insertions during the course of the mammoth’s evolution. The researchers note that their findings have implications for international efforts to resurrect extinct species, including the woolly mammoth. The study was published in the journal iScience by researchers at the Centre for Palaeogenetics in Stockholm, a collaboration between Stockholm University and the Swedish Museum of Natural History.

One of the most widely discussed methods to resurrect extinct species is to use genome editing techniques such as Crispr-Cas9 to insert key gene variants from an extinct species into a genome from its living relative. However, the results in this new study indicate that one might also need to remove certain genes to preserve important biological traits while reconstructing extinct genomes.

“Editing the genome of a living species to mimic that from an extinct relative was never going to be easy, and these new findings certainly illustrate the complexity and difficulties that lie ahead”, says Love Dalén, a professor of evolutionary genomics at the Centre for Palaeogenetics.

New research "UNCOVERS" hidden objects in high resolution

Target objects and the images of them created with UNCOVER NLOS technology.
Credit: Caltech

Imagine driving home after a long day at work. Suddenly, a car careens out of an obscured side street and turns right in front of you. Luckily, your autonomous car saw this vehicle long before it came within your line of sight and slowed to avoid a crash. This might seem like magic, but a novel technique developed at Caltech could bring it closer to a reality.

With the advent of autonomous vehicles, advanced spacecraft, and other technologies that rely on sensors for navigation, there is an ever-increasing need for advanced technologies that can scan for obstacles, pedestrians, or other objects. But what if something is hidden behind another object?

In a paper recently published in the journal Nature Photonics, Caltech researchers and their colleagues describe a new method that essentially transforms nearby surfaces into lenses that can be used to indirectly image previously obscured objects.

The technology, developed in the laboratory of Changhuei Yang, Thomas G. Myers Professor of Electrical Engineering, Bioengineering, and Medical Engineering; and Heritage Medical Research Institute investigator, is a form of non-line-of-sight (NLOS) sensing—or sensing that detects an object of interest outside of the viewer's line of sight. The new method, dubbed UNCOVER, does this by using nearby flat surfaces, such as walls, like a lens to clearly view the hidden object.