How a CRISPR Protein Might Yield New Tests for Many Viruses

In this illustration based on cryo-electron microscope images, a Cas12a2 protein unzips a DNA double helix, allowing it to cut the single strands of DNA (blue and green).
Illustration Credit: Jack Bravo/University of Texas at Austin

In a first for the genetic toolset known as CRISPR, a recently discovered protein has been found to act as a kind of multipurpose self-destruct system for bacteria, capable of degrading single-stranded RNA, single-stranded DNA and double-stranded DNA. With its abilities to target so many types of genetic material, the discovery holds potential for the development of new inexpensive and highly sensitive at-home diagnostic tests for a wide range of infectious diseases, including COVID-19, influenza, Ebola and Zika, according to the authors of a new study in the journal Nature.

Using a high-resolution imaging technique called cryo-EM, the team discovered that when this protein, named Cas12a2, binds to a specific sequence of genetic material from a potentially dangerous virus, called a target RNA, a side portion of Cas12a2 swings out to reveal an active site, similar to a sprung-open switchblade knife. Then, the active site starts to indiscriminately cut any genetic material it comes into contact with. The researchers discovered that, with a single mutation to the Cas12a2 protein, the active site degrades only single-stranded DNA—a feature especially useful in developing new diagnostics tailored for any of a wide range of viruses.

Major Breakthrough as Scientists Sequence the Genomes of Endangered Sharks

Hammerhead Shark
Photo Credit: David Clode

The first-ever chromosome-level genome sequences completed for great hammerhead and shortfin mako sharks have shown that both species have experienced major population declines over a 250,000-year history. Low genetic diversity and signs of inbreeding add a layer of concern to the management of Critically Endangered great hammerhead sharks, whose populations have been in freefall recently due to overfishing for their highly valued fins. In contrast, with a larger effective population size (the ideal breeding population size) in the past and higher genetic diversity, shortfin mako sharks appear equipped to be more resilient to rapid environmental change: that is, if the current fishing pressure on them is substantially reduced.

“With their whole genomes deciphered at high resolution we have a much better window into the evolutionary history of these endangered species,” said Mahmood Shivji, Ph.D., professor at Nova Southeastern University’s (NSU) Halmos College of Arts and Sciences and director of the Save Our Seas Foundation Shark Research Center and NSU’s Guy Harvey Research Institute.

It’s a startling image that describes a milestone in conservation science for sharks. Shivji, Michael Stanhope, Ph.D., from Cornell University’s College of Veterinary Medicine and their collaborators have glanced back in history by sequencing to chromosome level the genomes (entire genetic blueprint) of great hammerhead and shortfin mako sharks. Their DNA timeline shows that their populations have declined substantially over 250,000 years. What the scientists have also found is worrying: great hammerhead sharks have low genetic variation, which makes them less resilient to adapting to our rapidly changing world. The species also shows signs of inbreeding, an issue that can lower the ability of its populations to survive.

Serpent in the sky captured with ESO telescope

The Sh2-54 nebula in the infrared with VISTA
This image of the spectacular Sh2-54 nebula was taken in infrared light using ESO’s VISTA telescope at Paranal Observatory in Chile. The clouds of dust and gas that are normally obvious in visible light are less evident here, and in this light, we can see the light of the stars behind the nebulae now piercing through. 
Image Credit: ESO/VVVX

A myriad of stars is revealed behind the faint orange glow of the Sh2-54 nebula in this new infrared image. Located in the constellation Serpens, this stunning stellar nursery has been captured in all its intricate detail using the Visible and Infrared Survey Telescope for Astronomy (VISTA) based at ESO’s Paranal Observatory in Chile.

When the ancients looked up at the night sky, they saw random patterns in the stars. The Greeks, for instance, named one of these “constellations” Serpens, because of its resemblance to a snake. What they wouldn’t have been able to see is that at the tail end of this constellation there is a wealth of stunning astronomical objects. These include the Eagle, the Omega and the Sh2-54 nebulae; the last of these is revealed, in a new light, in this spectacular infrared image.

Nebulae are vast clouds of gas and dust from which stars are born. Telescopes have allowed astronomers to identify and analyze these rather faint objects in exquisite detail. The nebula shown here, located about 6000 light-years away, is officially called Sh2-54; the “Sh” refers to the US astronomer Steward Sharpless, who catalogued more than 300 nebulae in the 1950s.

Common Fatty Acid Contributes to Temperature and Pain Sensitivity in Psoriasis Plaques

Photo Credit: Eszter Miller

A common fatty acid found in the Western diet breaks down into compounds that contribute to increased temperature and pain – but not itch – sensitivity in psoriatic lesions. The finding could lead to better understanding of how lipids communicate with sensory neurons, and potentially to improved pain and sensitivity treatments for psoriasis patients.

Linoleic acid is a fatty acid found in vegetable oils, nuts and seeds, and is one of the predominant fatty acids found in the Western diet. Metabolites from linoleic acid – the products formed when the body breaks it down through digestion – play a role in skin barrier function.

“We noticed high levels of two types of lipids derived from linoleic acid in psoriatic lesions,” says Santosh Mishra, associate professor of neuroscience at North Carolina State University and corresponding author of the research. “That led us to wonder whether the lipids might affect how sensory neurons in these lesions communicate. We decided to investigate whether their presence could be related to the temperature or pain hypersensitivity that many psoriasis patients report.”

Zebrafish testing identifies a gene potentially at the root of domestication

Zebrafish
Photo Credit: Petr Kuznetsov

The research, published in iScience, looked at genetically modified zebrafish that fail to make the baz1b protein. The results suggest the gene is not only at the cornerstone of physical and behavioral changes in the fish and other domesticated species, but potentially also human beings’ social relationships.

Domesticated species - such as dogs and cats - show genetic differences compared to their wild type counterparts, including variation in the baz1b gene. These genetic changes correlate with physical and behavioral traits including smaller facial features such as skulls and teeth, as well as being more sociopositive, less aggressive, and having less fear.

However, studies have also suggested that modern humans domesticated themselves after they split from their extinct relatives, Neanderthals and Denisovans. In doing so, we experienced similar physical and behavioral changes.

Those changes have all been linked to the fact that domesticated animals have fewer of a certain type of stem cell, called neural crest stem cells.

The research led by the Queen Mary team builds on this by studying the impact of removing baz1b gene function, and the impact of doing so on neural crest development and social behavior.

Was That Explosion Chemical or Nuclear?

From left to right: Tim Johnson, Hunter Knox, and Harry Miley bring together different perspectives to better detect underground nuclear explosions. 
Composite Image Credit: by Shannon Colson | Pacific Northwest National Laboratory

If an underground explosion occurs anywhere in the world, there is a good chance that a seismologist can pinpoint it. However, they won’t necessarily be able to tell you what kind of explosion had occurred—whether it is chemical or nuclear in nature. New research from Pacific Northwest National Laboratory (PNNL) scientists makes detecting nuclear explosions easier.

“To a seismologist, chemical and nuclear explosions look identical,” said Harry Miley, Laboratory Fellow and physicist in the National Security Directorate at PNNL. “Radionuclide detection technologies, like the PNNL-developed Xenon International and Radionuclide Aerosol Sampler/Analyzer, known as RASA, can discriminate between the two by detecting radioactive atoms that are created in nuclear explosions. However, we have very little scientific understanding of the geologic containment of these atoms following an explosion.”

When an underground explosion occurs, gases travel through fractures in the ground and escape into the atmosphere. Instruments such as Xenon International and RASA can then detect radionuclide gases, but their chemical signatures may be greatly affected by rock damage that the gases must pass through.

Self-powered, printable smart sensors created from emerging semiconductors could mean cheaper, greener Internet of Things

Simon Fraser University professor Vincenzo Pecunia
Photo Credit: Courtesy of Simon Fraser University

Creating smart sensors to embed in our everyday objects and environments for the Internet of Things (IoT) would vastly improve daily life—but requires trillions of such small devices. Simon Fraser University professor Vincenzo Pecunia believes that emerging alternative semiconductors that are printable, low-cost and eco-friendly could lead the way to a cheaper and more sustainable IoT.

Leading a multinational team of top experts in various areas of printable electronics, Pecunia has identified key priorities and promising avenues for printable electronics to enable self-powered, eco-friendly smart sensors. His forward-looking insights are outlined in his paper published on Dec. 28 in Nature Electronics.

“Equipping everyday objects and environments with intelligence via smart sensors would allow us to make more informed decisions as we go about in our daily lives,” says Pecunia. “Conventional semiconductor technologies require complex, energy-intensity, and expensive processing, but printable semiconductors can deliver electronics with a much lower carbon footprint and cost, since they can be processed by printing or coating, which require much lower energy and materials consumption.”

Movement throttles insulin production

The figure shows the relationships between movement and regulation of the insulin-producing cells in the fruit fly.
Illustration Credit: Sander Liessem / University of Würzburg

If a fruit fly starts to run or fly, its insulin-producing cells are immediately inhibited. This could explain why exercise promotes health.

Insulin is an essential hormone for humans and many other living things. Its most well-known task is to regulate sugar metabolism. How it does this job is well researched. Much less is known about how the activity of the insulin-producing cells and consequently the release of insulin is controlled.

A team from the Biozentrum of the Julius Maximilians University (JMU) Würzburg is now providing news on this question in the journal Current biology in front. The group of Dr. used as the object of investigation. Jan Ache the fruit fly Drosophila melanogaster. Interestingly, this fly also releases insulin after a meal - but the hormone does not come from the pancreas like in humans, but from nerve cells in the brain.

Chip Circuit for Light Could Be Applied to Quantum Computations

Future versions of the new photonic circuits will feature low-loss waveguides—the channels through which the single photons travel--some 3 meters long but tightly coiled to fit on a chip. The long waveguides will allow researchers to more precisely choose the time intervals (Δt) when photons exit different channels to rendezvous at a particular location.
Illustration Credit: NIST

The ability to transmit and manipulate the smallest unit of light, the photon, with minimal loss, plays a pivotal role in optical communications as well as designs for quantum computers that would use light rather than electric charges to store and carry information.

Now, researchers at the National Institute of Standards and Technology (NIST) and their colleagues have connected on a single microchip quantum dots — artificial atoms that generate individual photons rapidly and on-demand when illuminated by a laser — with miniature circuits that can guide the light without significant loss of intensity.

To create the ultra-low-loss circuits, the researchers fabricated silicon- nitride waveguides—the channels through which the photons traveled—and buried them in silicon dioxide. The channels were wide but shallow, a geometry that reduced the likelihood that photons would scatter out of the waveguides. Encapsulating the waveguides in silicon dioxide also helped to reduce scattering.

Researchers Demonstrate New Strain Sensors in Health Monitoring, Machine Interface Tech

Image Credit: Shuang Wu.

Researchers at North Carolina State University have developed a stretchable strain sensor that has an unprecedented combination of sensitivity and range, allowing it to detect even minor changes in strain with greater range of motion than previous technologies. The researchers demonstrated the sensor’s utility by creating new health monitoring and human-machine interface devices.

Strain is a measurement of how much a material deforms from its original length. For example, if you stretched a rubber band to twice its original length, its strain would be 100%.

“And measuring strain is useful in many applications, such as devices that measure blood pressure and technologies that track physical movement,” says Yong Zhu, corresponding author of a paper on the work and the Andrew A. Adams Distinguished Professor of Mechanical and Aerospace Engineering at NC State.

“But to date there’s been a trade-off. Strain sensors that are sensitive – capable of detecting small deformations – cannot be stretched very far. On the other hand, sensors that can be stretched to greater lengths are typically not very sensitive. The new sensor we’ve developed is both sensitive and capable of withstanding significant deformation,” says Zhu. “An additional feature is that the sensor is highly robust even when over-strained, meaning it is unlikely to break when the applied strain accidently exceeds the sensing range.”