Mastodon Scientific Frontline: Improved Mineralized Material Can Restore Tooth Enamel

Friday, September 16, 2022

Improved Mineralized Material Can Restore Tooth Enamel

Scientists tested the effectiveness of the new enamel coating on real healthy teeth.
Photo credit: Danil Ilyukhin

Scientists have perfected hydroxyapatite, a material for mineralizing bones and teeth. By adding a complex of amino acids to hydroxyapatite, they were able to form a dental coating that replicates the composition and microstructure of natural enamel. Improved composition of the material repeats the features of the surface of the tooth at the molecular and structural level, and in terms of strength surpasses the natural tissue. The new method of dental restoration can be used to reduce the sensitivity of teeth in case of abrasion of enamel or to restore it after erosion or improper diet. The study and experimental results are published in Results in Engineering.

"Tooth enamel has a protective function, but unfortunately, its integrity can be destroyed by, for example, abrasion, erosion or microfractures. If the surface of the tissue is not repaired in time, the enamel lesion will affect the dentin and then the pulp of the tooth. Therefore, it is necessary to restore the enamel surface to a healthy level or build up additional layers on the surface if it has become very thin. We have created a biomimetic (i.e., mimicking natural) mineralized layer whose nanocrystals replicate the ordering of apatite nanocrystals of tooth enamel. We also found out that the designed layer of hydroxyapatite has increased nanohardness that exceeds that of native enamel," says Pavel Seredin, Leading Specialist of Research and Education Center "Nanomaterials and Nanotechnologies", Ural Federal University, Head of the Department of Solid State Physics and Nanostructures at Voronezh State University.

Hydroxyapatite is a compound that is a major component of human bones and teeth. Scientists selected a complex of polyfunctional organic and polar amino acids, including, for example, lysine, arginine, and histidine, which are important for the formation and repair of bone and muscle structures. The chosen amino acids made it possible to obtain hydroxyapatite, which is morphologically completely similar to apatite (the main component of tissues) of dental enamel. The researchers also described the conditions of the environment in which the processes of binding hydroxyapatite to the dental tissue should occur. Only if these conditions are met is it possible to fully reproduce the structure of natural enamel.

"Traditionally in dentistry, composite restorative materials are used in enamel restoration. To increase the bonding efficiency of enamel and composite, the restoration technique involves acid etching of the enamel beforehand. The etching products left behind may not always have a positive effect on the bonding of enamel and synthetic materials. To reproduce the enamel layers with biomimetic techniques, we neutralized the media and removed the etching products using calcium alkali. In this way we improved the binding of the new hydroxyapatite layers," explains Pavel Seredin.

The formation of a mineralized layer with properties resembling those of natural hard tissue was confirmed by field emission electron and atomic force microscopy as well as by chemical imaging of surface areas using Raman microspectroscopy. The study was conducted on healthy teeth to eliminate the influence of extraneous factors on the resulting layer and to be able to compare the results with healthy teeth. Next, the researchers will tackle the challenge of repairing larger defects, which can be of varying nature from the initial stages of caries to cracks and volumetric fractures.

The joint research was conducted by scientists from the Research and Education Center "Nanomaterials and Nanotechnologies" of Ural Federal University, Voronezh State University, Voronezh State Medical University, Al-Azhar University, and the National Research Center (Egypt). This work was supported by a grant from the Russian Science Foundation (Project № 21-75-10005).

Reference

Tooth enamel consists mainly of an inorganic substance (biological apatite, about 95% by weight), an organic component (collagen fibers, 1-1.5%) and water (4%). Dental composite materials do not have the ability to bind physically and chemically to the dental tissues on their own. Therefore, additional materials must be used to create a strong bond between the composite and the tooth tissue. To increase the bonding effectiveness of enamel and composite, dental restoration technique involves acidic enamel pretreatment with a liquid or gel based on phosphoric acid (10-37%) or maleic acid (10%).

Hydroxyapatite is the main inorganic component of bone tissue, mineralized tissues of humans and animals, due to which it is widely used in various fields of medicine such as cosmetology, orthopedics, dentistry. Hydroxyapatite is produced chemically, with the participation of a secondary source of calcium that is the egg shells of birds on the microelement composition, structure and size factor is similar to the apatite of dental enamel. This method of synthesizing hydroxyapatite is easy to use and economically feasible.

Source/Credit: Ural Federal University

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