New study examines the relationship between the rate of wound healing, the circadian rhythm, and ‘hair’ on cells

Fibroblasts need to accumulate at the wound site when repairing wounds such as skin wounds. We found that fibroblasts with long primary cilia migrate slower to the wound site than fibroblasts with short primary cilia.
Illustration Credit: Ryota Nakazato/Hiroshima University

We’re all familiar with our body’s internal clock: it gives us cues on when to wake and when to rest, but it also can determine the rate and time of day at which your body most effectively heals wounds.

Nearly every organism on Earth follows a natural circadian rhythm that is coded by your cell’s clock genes, which do exactly as you suspect from the name: regulate your body’s rhythm on a 24-hour basis. Most cells in mammalian bodies have cilia of some sort, which are hair-like structures that perform a variety of functions such as movement for motile cilia and aiding in structure in function for non-motile, or primary, cilia. The primary cilia also act as a sensory organ for the cell, a function which has illuminated the primary cilia’s potential role in the healing process and how bodies heal at a different rate according to our circadian rhythm. In this research, the role of the primary cilia, biological clock and wound healing is explored.

Ural Scientists Assessed How Vegetation Changes in the Mountains

As scientists explain, the woody vegetation of high mountains is a sensitive indicator of climate change.
 Photo Credit: Pavel Yelfimov

Specialists of the Ural Carbon test site, researchers from the Ural Federal University (UrFU) with colleagues from the Ural State Forestry Technical University, have proposed a methodology for automated mapping of high mountain woody vegetation. The methodology is based on the comparison of data from archival aerial images and modern satellite images, RIA Novosti reported. The work of researchers will help to objectively assess the peculiarities of the appearance of new trees on unforested territory depending on the influence of the environment. The description of the methodology is published in the journal Forests.

"Woody vegetation of high mountains, near its upper limit of growth, is a sensitive indicator of climate change. Therefore, mountain ranges in different regions of the Earth are considered as areas for studying the early vegetation response to regional climate change. The aim of our work was twofold. The first part involves demonstrating an effective method of automated mapping of vegetation cover units: forest, sparse woodland, open stand, and tundra with freestanding trees developed by us, and to analyze changes occurring in plant communities under the conditions of modern climate warming near the upper limit of tree growth. The second part includes evaluating the efficiency of the method on the example of assessing the expansion of Siberian larch into mountain tundra in the Polar Urals over the past 50 years," explains Valery Fomin, Vice-Rector for Research and Innovation at Ural State Forestry Technical University and researcher at UrFU.

Shock wave photographed passing through a single cell

Images of an underwater shock wave moving through a HeLa cell.
Using this new technology, the researchers could see the difference between how the shock wave moved inside and outside of a cell submerged in water. They noted that the results suggested that the cell structure shifts with the visualized wavefront position (shown in the red/ orange line in the image).
 Image Credit: © 2023 Saiki et al. University of Tokyo

A microscopic shock wave has been photographed passing through a single biological cell, thanks to a new photography technique. Nanosecond photography uses ultrafast electronic cameras to take images at the speed of a billionth of a second. However, image quality and exposure time are typically limited. Now, a team led by researchers at the University of Tokyo has achieved superfine images taken over multiple timescales at high-speed using a system they named spectrum circuit. Spectrum circuit bridges the gap between optical imaging and conventional electronic cameras, enabling photography at ultrafast speeds with less blur and more accuracy. This technology has potential applications for science, medicine and industry.

You’re waiting with your camera for just the right moment. Suddenly, your subject speeds by and you’ve barely clicked the shutter. Missed it. Timing can be everything in photography and capturing images at high speed poses a particular challenge. But thanks to advances in camera technology, these days we can see the world like never before. Whether it’s the sweat on a racing cyclist’s brow, the focus in the eyes of a swooping falcon or, with this latest improvement in nanosecond photography, the movement of a shock wave passing through a microscopic single cell at high speed.

Light color is less important for the internal clock than originally thought

To what extent does the color of light influence our internal clock and sleep? The results of a study conducted in Basel shed light on this.
Photo Credit: Josh Hild

Light in the evening is thought to be bad for sleep. However, does the color of the light play a role? Researchers from the University of Basel and the Technical University of Munich (TUM) compared the influence of different light colors on the human body. The researchers’ findings contradict the results of a previous study in mice.

Vision is a complex process. The visual perception of the environment is created by a combination of different wavelengths of light, which are decoded as colors and brightness in the brain. Photoreceptors in the retina first convert the light into electrical impulses: with sufficient light, the cones enable sharp, detailed, and colored vision. Rods only contribute to vision in low light conditions allowing for different shades of grey to be distinguished but leaving vision much less precise. The electrical nerve impulses are finally transmitted to ganglion cells in the retina and then via the optic nerve to the visual cortex in the brain. This region of the brain processes the neural activity into a colored image.

Monash researchers discover key to epithelial cell growth

L-R: Dr Benjamin Kroeger and Professor Kieran Harvey
Photo Credit: Courtesy of Monash University

Monash researchers have discovered a new way that epithelial cells, which form layers in organs such as the skin and stomach, attach to one another, and how they perceive growth signals at these attachments, helping them form tissues of the right size and shape.

Epithelial cells cover the surfaces of most organs in the body and must adhere to each other to form both a protective and permeable barrier. They are exquisitely designed to both be tightly sealed against pathogens such as bacteria, and to also allow the transport of salts, fluids, and nutrients.

Researchers, led by Professor Kieran Harvey and first author Dr Benjamin Kroeger, at the Monash University Biomedicine Discovery Institute have discovered a new way by which epithelial cells adhere to each other in the vinegar fly, Drosophila. The study is published in the journal, Developmental Cell.

Previous work from Professor Harvey and others led to the discovery of an important organ growth control pathway, called Hippo. First discovered in Drosophila, the Hippo pathway does effectively the same job in mammals and controls the size of different organs such as the liver and heart. The Hippo pathway is also important for human diseases as it is mutated in multiple epithelial cancers.

Joint research project on groundwater: searching for underground pollutants

A copepod is a crustacean barely a millimeter in size which lives in groundwater. The presence of such animals is an indicator of good water quality.
Photo Credit: Sabrina Schiwy, Goethe University Frankfurt

The joint project, recently launched under the name “gwTriade," involves six scientific institutes with Goethe University Frankfurt as the coordinator, which are investigating groundwater quality in Germany. This is the first time the triad approach has been applied to combine chemical analyses and methods revealing how pollutants entering the groundwater affect the ecosystem there – called effect-based methods. The project aims to develop a concept that water suppliers and nature conservation authorities can use in the future to examine and assess the groundwater quality themselves. The gwTriade project is funded by the Federal Ministry of Education and Research. 

The effects of climate change pose an ever-greater threat to our groundwater because more frequent and longer periods of drought reduce groundwater levels. Groundwater is therefore already supplemented with surface water in conurbations like the Rhine-Main area. This surface water often contains treated wastewater that may add pollutants to the groundwater. More frequent heavy rainfalls – another consequence of climate change – lead to large quantities of pollutants entering the groundwater. As a result, over one third of all groundwater bodies in Germany fail to achieve good chemical status. The European Water Framework Directive establishes the legal framework for assessing the quality of groundwater. However, a “huge amount of investigation" into the groundwater quality is still required, according to Professor Henner Hollert from the Institute of Ecology, Diversity and Evolution at Goethe University Frankfurt. Chemical analyses have identified at least some of the pollutants in the groundwater, including drugs, pesticides and perfluoroalkyl substances (PFAS), which originate from the wastewater, traffic or agriculture. “What we don't have at all is effect-based data, i.e. data about how the pollutants impact life in the groundwater ecosystem and also human health. We already know a lot about surface water, but not about the groundwater." 

New COVID vaccine induces good antibody response to mutated viral variants

Photo Credit: CDC

Researchers at Karolinska Institutet and Danderyd Hospital have followed recipients of the new updated COVID-19 vaccine and analyzed the antibody response to different SARS-CoV-2 variants. The results show a surprisingly strong response to the now dominant and highly mutated Omicron variants.

The ongoing COMMUNITY study, which was launched in the spring of 2020 with the regular testing of 2,149 members of the Danderyd Hospital staff, has recently published the results of this autumn’s leg of the study. Twenty-four participants were recorded in this study, the majority of whom were over 64 and had received four or five previous vaccine doses. The article has been peer-reviewed and accepted for publication in the scientific journal The Lancet Infectious Diseases, and is accessible prior to publication on the preprint server, bioRxiv.

Octopus DNA solves mystery of ice sheet’s past

Octopus, probably Pareledone species, from 500m depth on the Bellingshausen Sea continental shelf.
Photo Credit: British Antarctic Survey

Scientists, including from British Antarctic Survey, have used octopus DNA to discover that the West Antarctic Ice Sheet (WAIS) likely collapsed during the Last Interglacial period around 120,000 years ago – when the global temperatures were similar to today.

This provides the first empirical evidence that the tipping point of this ice sheet could be reached even under the Paris Agreement targets of limiting warming to 1.5-2oC.

The study, published in the journal Science, was led by Professor Jan Strugnell, Chief Investigator, and Dr Sally Lau, Postdoctoral Research Fellow from ARC Securing Antarctica’s Environmental Future at James Cook University.

Octopus, probably Pareledone species, from 500m depth on the Bellingshausen Sea continental shelf. BAS.

Artificial intelligence unravels mysteries of polycrystalline materials

Researchers used 3D model created by AI to understand complex polycrystalline materials that are used in our everyday electronic devices.
Illustration Credit: Kenta Yamakoshi

Researchers at Nagoya University in Japan have used artificial intelligence to discover a new method for understanding small defects called dislocations in polycrystalline materials, materials widely used in information equipment, solar cells, and electronic devices, that can reduce the efficiency of such devices. The findings were published in the journal Advanced Materials.  

Almost every device that we use in our modern lives has a polycrystal component. From your smartphone to your computer to the metals and ceramics in your car. Despite this, polycrystalline materials are tough to utilize because of their complex structures. Along with their composition, the performance of a polycrystalline material is affected by its complex microstructure, dislocations, and impurities. 

A major problem for using polycrystals in industry is the formation of tiny crystal defects caused by stress and temperature changes. These are known as dislocations and can disrupt the regular arrangement of atoms in the lattice, affecting electrical conduction and overall performance. To reduce the chances of failure in devices that use polycrystalline materials, it is important to understand the formation of these dislocations. 

How technology and economics can help save endangered species

The gray wolf is among the animals protected by the Endangered Species Act.
Image Credit: Oregon Department of Fish & Wildlife
(CC BY-SA 2.0)

A lot has changed in the world since the Endangered Species Act (ESA) was enacted 50 years ago in December 1973.

Two researchers at The Ohio State University were among a group of experts invited by the journal Science to discuss how the ESA has evolved and what its future might hold.

Tanya Berger-Wolf, faculty director of Ohio State’s Translational Data Analytics Institute, led a group that wrote on “Sustainable, trustworthy, human-technology partnership.”  Amy Ando, professor and chair of the university’s Department of Agricultural, Environmental, and Development Economics, wrote on “Harnessing economics for effective implementation.”

Berger-Wolf and her colleagues wrote, “We are in the middle of a mass extinction without even knowing all that we are losing and how fast.” But technology can help address that.

For example, they note the value of tools like camera traps that survey animal species and smartphone apps that allow citizen scientists to count insects, identify bird songs and report plant observations.

Inside the Matrix: Nanoscale Patterns Revealed Within Model Research Organism

Super-resolution microscopy reveals two roundworm collagens labeled in red and green.
Image Credit: Courtesy of University of California San Diego

Species throughout the animal kingdom feature vital interfaces between the outermost layers of their bodies and the environment. Intricate microscopic structures—featured on the outer skin layers of humans, as one example—are known to assemble in matrix patterns.

But how these complex structures, known as apical extracellular matrices (aECMs) are assembled into elaborately woven architectures has remained an elusive question.

Now, following years of research and the power of a technologically advanced instrument, University of California San Diego scientists have unraveled the underpinnings of such matrices in a tiny nematode. The roundworm Caenorhabditis elegans has been studied extensively for decades due to its transparent structure that allows researchers to peer inside its body and examine its skin.

Described in the journal Nature Communications, School of Biological Sciences researchers have now deciphered the assemblage of aECM patterns in roundworms at the nanoscale. A powerful, super-resolution microscope helped reveal previously unseen patterns related to columns, known as struts, that are key to the proper development and functioning of aECMs.

“Struts are like tiny pillars that connect the different layers of the matrix and serve as a type of scaffolding,” said Andrew Chisholm, a professor in the School of Biological Sciences and the paper’s senior author.

The future of canine stem cell therapy: unprecedented, painless, and feeder-free

Generating canine induced pluripotent stem cells (iPSCs) without using feeder cells   Scientists created canine iPSCs from urine-derived cells with great efficiency     
Illustration Credit: Shingo Hatoya, Osaka Metropolitan University

Dog owners may need to learn to appreciate their best friend’s urine. Scientists at Osaka Metropolitan University have devised an efficient, non-invasive, and pain-free method to reprogram canine stem cells from urine samples, bringing furry companions one step closer to veterinary regenerative treatment.

Induced pluripotent stem cells (iPSCs) have been widely employed in studies on human generative medicine. With the growing importance of advanced medical care for dogs and cats, there is an expectation that new therapies utilizing iPSCs will be developed for these companion animals, just as they have been for humans. Unfortunately, canine somatic cells exhibit lower reprogramming efficiency compared to those of humans, limiting the types of canine cells available for generating iPSCs. IPSC induction often involves using feeder cells from a different species. However, considering the associated risks, minimizing xenogeneic components is often advisable, signifying the need to improve the efficiency of reprogramming various types of canine cells in dogs without using feeder cells.

Moderation surpasses excess

DYRK1A bound to FAM53C in the cytoplasm is less active. DYRK1A not bound to FAM53C in the nucleus is highly active. Functional abnormalities cause various neuropsychiatric developmental and functional disorders. 
Illustration Credit: KyotoU/Gakuji Tobiyama/Yoshihiko Miyata

Down syndrome, a congenital disorder stemming from abnormal cell division and differentiation, is most common in newborns fated to neurodevelopmental delays and other health complications.

The genetic defect causes the dysfunction of the protein kinase DYRK1A, which is encoded on chromosome 21 and is deeply associated with both Down syndrome and autism spectrum disorder. DYRK1A has attracted attention as a target molecule for treating various diseases, but specific cellular mechanisms regulating the enzyme DYRK1A have yet to be made clear.

Now, researchers at Kyoto University have identified the FAM53C protein and its DYRK1A-inhibiting effect that keeps the protein kinase inactive inside the cytoplasm.

Artery calcification more common in night owls

Artery calcification is almost twice as common in night owls compared to early birds, according to a study from the University of Gothenburg, Sweden. Circadian rhythm appears to be particularly important for the heart and blood vessels during the early stages of the disease.

Artery calcification, or atherosclerosis as it is also known, involves fatty deposits accumulating on the inside of the arteries, making it harder for blood to pass through. The disease develops over a very long period of time, and is not noticed until it leads to angina, blood clots, heart attack, or stroke. Previous research has shown that people with late-night habits have an increased risk of cardiovascular disease, but this is the first study to show how circadian rhythm specifically affects artery calcification.

Coronary artery calcification

The study, which has been published in the journal Sleep Medicine, involved 771 men and women aged between 50 and 64, all of whom are part of the larger population study SCAPIS. The degree of artery calcification in the heart’s coronary arteries was examined using computer tomography. Participants themselves indicated their so called chronotype on a five-point scale: extreme morning type, morning type to some extent, neither morning nor evening type, evening type to some extent, or extreme evening type.

Common insect species are suffering the biggest losses

The invasive Asian Ladybeetle (Harmonia axyridis)
Photo Credit: Melani Marfeld

Insect decline is being driven by losses among the locally more common species, according to a new study published in Nature. Led by researchers at the German Centre for Integrative Biodiversity Research (iDiv) and the Martin Luther University Halle-Wittenberg (MLU), the meta-analysis of 923 locations around the world notes two significant trends: Species with the most individuals are disproportionately decreasing in number, and no other species have increased to the high numbers previously seen. This likely explains the frequent observation that there are fewer insects around now than ten, twenty, or thirty years ago.

Researchers at iDiv looked at long-term trends of land-based insects, such as beetles, moths, and grasshoppers, and found that decreases in the number of the formerly most common species have contributed most to local insect declines. Common or abundant insect species are those species that are locally found in the highest numbers, but which species these are differ among locations. The study’s findings challenge the idea that changes in insect biodiversity result from rarer species disappearing.

The study follows the recent sounding of alarm bells about insect loss, as researchers note dramatic declines in the total number of insects in many parts of the world. However, little is known about the general trends among locally rare and abundant species over long periods. "It was obvious this needed exploring," says Roel van Klink, lead author of the study and senior scientist at iDiv and MLU. "We had to know whether observations about declines in total abundances of insects differed among common and rare species, and how this translated into changes in the overall insect diversity."