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| Strengthened Saccharomyces cerevisiae This common yeast is a strong contender for replacing petroleum in 2,3-butanediol production. Image Credit: Osaka Metropolitan University |
As fossil fuels rise in cost and green initiatives gain traction, alternative methods for producing useful compounds using microorganisms have the potential to become sustainable, environmentally friendly technologies.
One such process involves the common bread yeast, Saccharomyces cerevisiae (S. cerevisiae), to produce 2,3-butanediol (2,3-BDO), an organic compound often used in pharmaceuticals and cosmetics. However, this yeast has a low tolerance for 2,3-BDO under high concentrations, which leads to a decline in its production ability and hinders the mass commercialization of this method.
To work around this, Associate Professor Ryosuke Yamada’s team at Osaka Metropolitan University’s Graduate School of Engineering attempted to enhance S. cerevisiae by introducing mutations into the genomic DNA. The researchers engineered four altered strains and subjected them to ethanol, heat, and low pH stressors.
As a result, they successfully obtained the YPH499/Co58 mutant strain that proliferates 122 times more than the parent strain in a high-concentration 2,3-BDO environment. Furthermore, gene expression analysis confirmed that the activation of genes associated with proteasome, peroxisome, the tricarboxylic acid cycle, mitochondria, and transcriptional regulation played a crucial role in 2,3-BDO tolerance.
“The technology used to mutate the yeast’s genomic DNA is a highly effective base technology for enhancing its capabilities,” stated Professor Yamada. “This method could contribute to creating a sustainable society by not only producing 2,3-BDO but also facilitating the development of robust microorganisms capable of efficiently producing other useful substances.”
Funding: This study was supported by the GteX Program of Japan (Grant number: JPMJGX23B4).
Published in journal: Applied Microbiology and Biotechnology
Title: Construction of yeast with extremely high 2,3-butanediol tolerance by introducing point and structural mutations and partial elucidation of the mechanism of 2,3-butanediol tolerance
Authors: Kaito Nakamura, Ryosuke Yamada, Rumi Sakaguchi, Takuya Matsumoto, and Hiroyasu Ogino
Source/Credit: Osaka Metropolitan University
Reference Number: btech121525_01
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